Spp1-mediated generalized transduction
Webtransferred to the NCIB3610 background using SPP1-mediated generalized transduction (Yasbin & Young, 1974). All strains are listed in Table 1. E. coli XL1-Blue (Stratagene) was used for plasmid constructions and transformation using standard techniques (Sambrook et al., 1989). Luria–Bertani (LB) broth was used to grow E. coli and Bacillus ... Web1 Sep 2024 · Bacillus subtilis SPP1 is a generalized transducing bacteriophage. Using this lytic phage as a model, we have analyzed how viral replication and recombination systems contribute to the transfer of...
Spp1-mediated generalized transduction
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Web%A Valero-Rello,Ana %A López-Sanz,María %A Quevedo-Olmos,Alvaro %A Sorokin,Alexei %A Ayora,Silvia %D 2024 %J Frontiers in Microbiology %C %F %G English %K horizontal gene … Web16 Jul 2024 · By Michael Greenwood, M.Sc. Reviewed by Sophia Coveney. Transduction is the biological process by which DNA is transferred into a cell with the aid of a viral vector. This may take place naturally ...
Web30 Nov 2005 · The Campbell integrant was then transduced to 3610 strain derivatives by SPP1 phage mediated transduction. To excise the plasmid, transductants were first propagated at the permissive temperature for plasmid replication (30°C) for 6 hours, transferred to 37°C and incubated for 3 hours prior to being dilution plated on LB … Web30 Nov 2024 · Generalized transduction remains an important genetic tool for fine-structure mapping, site-directed mutagenesis, and transposon-related genetic manipulation even in …
WebThe ΔpolY1:tet and ΔpolY2:kan mutations were moved onto the BG214 background by viral SPP1-mediated generalized transduction, as described [39]. ... Bacillus subtilis DisA helps to circumvent ... WebThe procapsid of the Bacillus subtilis bacteriophage SPP1 is formed by the major capsid protein gp13, the scaffolding protein gp11, the portal protein gp6, and the accessory …
Web1 May 2013 · When the SPP1 packaging signal was integrated onto the host genome, and SPP1-mediated generalized transduction was used as a tool to define a minimal …
Web14 Feb 1979 · The generalized transduction system mediated by SPP1 lysates was analyzed. After removal of contaminating PBS particles, SPP1 lysates retain the same levels of transducing activity. The SPP1 particles of normal size, shape and buoyant density carrying bacterial DNA, are vectors in a true transduction process. Next two dvd portablehttp://genesdev.cshlp.org/content/suppl/2005/11/30/19.24.3083.DC1/KearnsSuppMat.pdf two duties of the priest in ancient egyptWeb17 Feb 2024 · In mice, Aβ prompts perivascular macrophages to express the cytokine SPP1. SPP1 stimulates microglia to eat synapses. Knocking out SPP1 protects synapses in a … talk about my family英语作文WebBacteriophage (phage)-mediated generalized transduction is expected to contribute to the emergence of drug-resistant staphylococcal clones in various environments. In this study, novel phage S6 was isolated from sewage and used to test generalized transduction in human- and animal-derived staphylococci. Phage S6 was a novel type of giant ... two dvi portsWeb16 Oct 2024 · The mutated amyE::P hag-hag T209C spec locus was then transduced to the DS1677 ∆hag 3610 ancestral strain background via SPP1-mediated generalized phage … two dwarf planets clueWeb3 Feb 2024 · The positive clones are then used for allele replacement into B. subtilis strain NCIB3610 employing SPP1-mediated generalized transduction (see Subheading 3.1.3). … talk about my college lifeWebThe procapsid of the Bacillus subtilis bacteriophage SPP1 is formed by the major capsid protein gp13, the scaffolding protein gp11, the portal protein gp6, and the accessory protein gp7. The protein stoichiometry suggests a T = 7 symmetry for the SPP1 procapsid. Overexpression of SPP1 procapsid proteins in two d vector initialization in c++