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Sato รุ่นNSII …
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Supplementary Materials Materials and Methods Patient tissues …
WebThermo Scientific Mph1103I (NsiI) restriction enzyme recognizes ATGCA^T sites and cuts best at 37C in R buffer (Isoschizomers: EcoT22I, NsiI, Zsp2I).Thermo Scientific … Webvector (pCpGfree-promoter-Lucia (Invivogen)) using FastDigest BcuI (SpeI) and NsiI (Thermo Fisher Scientific). Constructs were transformed into E. coli containing PIR1 (One ShotTM PIR1 Chemically Competent E. coli, Invitrogen) and amplified using Qiaprep Spin MiniPrep Kit (Qiagen). WebThe restricting enzyme of Mph1103I (NsiI; Thermo Fisher Scientific) was used for the detection of A>C transversion polymorphism in the Cyp2C9*3 gene. The PCR‐RFLP mixture included 16μl of distilled water, 1μlofNsiI, 3μl of Buffer R (Thermo Fisher Scientific), and 10μl of PCR products so that the final volume was around 30μl. This lg cordless tower vacuum